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KMID : 0378019750180121540
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New Medical Journal
1975 Volume.18 No. 12 p.1540 ~ p.1550
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Cell biologic changes of the unfixed odontoblasts
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Abstract
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The author has studied the morphologic changes of the unfixed odontoblasts suspended is Hanks solution and diluted formcresol solution at interval 10, 30, and 60 minutes by the phase contrast microscope.
The odontoblasts were taken from the lower incisors of the adult male rats. The results were as follows.
1. In Hanks solution, nuclear membrane were thickened and the contrast of chromatin were increased. Cytoplasmic granules of odontoblasts showed the gradual increase of the phase dark contrast and aggregation-like appearance. Vacuoles in the cytoplasm were presented clearly in the periphery of the nucleus, and no change in 30 minutes.
2. In 0.25% formcresol solution, nucleus revealed pyknosis immediately after dropping, but karyorrhexis also followed with time, and the contrast of chromatin wre decreased gradually. Cytoplasmic granules of odontoblasts revealed the increased contrast immediately after dropping and aggregation-like appearance. Vacuoles in the cytoplasm of odontoblasts were in the periphery of nucleus.
3. In 0.5% formcresol solution, the changes were resembled to the appearances of 0.25 formcresol solution, except for appearing the thick-rim appearance of the cytoplasmic granules and blister formation on the periphery of the cytoplasmic membrane.
4. In 1.0% formcresol solution, it showed the same appearance as the 0.5% formcresol solution, except for showing the compressing appearances of the nucleus by vacuoles formation.
5. In 2.0% formcresol solution, nucleus revealed the severe karyorrhexis. The cytoplasmic granules showed the thick-rim appearances.
6. In 3.0% formcresol solution, nucleus revealed the severe karyorrhexis immediately after dropping, but occassionally karyolysis. The cytoplasmic granules showed the prominant thick-rim appearance immediately after dropping.
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